Description

Calculate read duplication rate

Input

name:type
description
pattern

meta:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end

]

bam:file

the alignment in bam format

*.{bam}

Output

name:type
description
pattern

seq_xls

meta:file

Read duplication rate determined from mapping position of read

*seq.DupRate.xls

*seq.DupRate.xls:file

Read duplication rate determined from mapping position of read

*seq.DupRate.xls

pos_xls

meta:file

Read duplication rate determined from sequence of read

*pos.DupRate.xls

*pos.DupRate.xls:file

Read duplication rate determined from sequence of read

*pos.DupRate.xls

pdf

meta:file

plot of duplication rate

*.pdf

*.pdf:file

plot of duplication rate

*.pdf

rscript

meta:file

script to reproduce the plot

*.R

*.r:file

script to reproduce the plot

*.R

versions

versions.yml:file

File containing software versions

versions.yml

Tools

rseqc
GPL-3.0-or-later

RSeQC package provides a number of useful modules that can comprehensively evaluate high throughput sequence data especially RNA-seq data.